f 10 × nebuffer r3 1 (New England Biolabs)
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F 10 × Nebuffer R3 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuffer+r3+1+10/NEBuffer+r3%2E1/pm40263707-80-18-21
Average 99 stars, based on 290 article reviews
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High Performance Liquid Chromatography:Article Title: Control of DNA replication in vitro using a reversible replication barrier. Article Snippet: .. • 5′ phosphorylated, HPLC/ polyacrylamide gel electrophoresis (PAGE)-purified oligonucleotide containing the DNA adduct of interest • Nb.BsmI (NEB, cat. no. R0706S) • T4 DNA ligase (NEB, cat. no. M0202S) • Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, cat. no. T1030L) • Monarch Plasmid Miniprep Columns (NEB, cat. no. T1017L) Polyacrylamide Gel Electrophoresis:Article Title: Control of DNA replication in vitro using a reversible replication barrier. Article Snippet: .. • 5′ phosphorylated, HPLC/ polyacrylamide gel electrophoresis (PAGE)-purified oligonucleotide containing the DNA adduct of interest • Nb.BsmI (NEB, cat. no. R0706S) • T4 DNA ligase (NEB, cat. no. M0202S) • Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, cat. no. T1030L) • Monarch Plasmid Miniprep Columns (NEB, cat. no. T1017L) Polymerase Chain Reaction:Article Title: Control of DNA replication in vitro using a reversible replication barrier. Article Snippet: .. • 5′ phosphorylated, HPLC/ polyacrylamide gel electrophoresis (PAGE)-purified oligonucleotide containing the DNA adduct of interest • Nb.BsmI (NEB, cat. no. R0706S) • T4 DNA ligase (NEB, cat. no. M0202S) • Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, cat. no. T1030L) • Monarch Plasmid Miniprep Columns (NEB, cat. no. T1017L) Plasmid Preparation:Article Title: Control of DNA replication in vitro using a reversible replication barrier. Article Snippet: .. • 5′ phosphorylated, HPLC/ polyacrylamide gel electrophoresis (PAGE)-purified oligonucleotide containing the DNA adduct of interest • Nb.BsmI (NEB, cat. no. R0706S) • T4 DNA ligase (NEB, cat. no. M0202S) • Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, cat. no. T1030L) • Monarch Plasmid Miniprep Columns (NEB, cat. no. T1017L) In Vitro:Article Title: Modulation of Equid Herpesvirus-1 Replication Dynamics In Vitro Using CRISPR/Cas9-Assisted Genome Editing Article Snippet: .. The in vitro cell-free cleavage assay was performed as follows: a 27 μL reaction mix was prepared containing 3 μL of 300 nM Cas9 protein (Invitrogen) diluted in 1× Diluent B (New England BioLabs, Ipswich, MA, USA), 3 μL of 300 nM sgRNAs, 3 μL of Cleavage Assay:Article Title: Modulation of Equid Herpesvirus-1 Replication Dynamics In Vitro Using CRISPR/Cas9-Assisted Genome Editing Article Snippet: .. The in vitro cell-free cleavage assay was performed as follows: a 27 μL reaction mix was prepared containing 3 μL of 300 nM Cas9 protein (Invitrogen) diluted in 1× Diluent B (New England BioLabs, Ipswich, MA, USA), 3 μL of 300 nM sgRNAs, 3 μL of |


